Wetting-Induced Budding of Vesicles in Contact with Several Aqueous Phases
نویسندگان
چکیده
Osmotic deflation of vesicles enclosing two liquid phases can lead to bulging of one of the phases from the vesicle body. This budding process is preceded by a complete to partial wetting transition of one of the liquid phases on the membrane and depends on the membrane tensions and the tension of the interface between the enclosed liquid phases. These tensions dominate in different morphology regimes, the crossover of which initiates the budding process. In addition, the degree of budding can be controlled by aspiration via micropipets. We also demonstrate that the budding direction can be reversed if there are two external phases in contact with the vesicle.
منابع مشابه
Intrinsic contact angle of aqueous phases at membranes and vesicles.
Biomimetic membranes exposed to several aqueous phases are studied theoretically. Specific examples are provided by lipid vesicles and phase separating polymer solutions. The theory reveals the existence of an intrinsic contact angle, theta(in), between the membranes and the aqueous phases. An explicit relation is derived by which this intrinsic angle can be determined from the effective contac...
متن کاملIntrinsic contact angle of aqueous phases at membranes and vesicles (preprint)
Biomimetic membranes exposed to several aqueous phases are studied theoretically. Specific examples are provided by lipid vesicles and phase separating polymer solutions. The theory reveals the existence of an intrinsic contact angle, θin, between the membranes and the aqueous phases. An explicit relation is derived by which this intrinsic angle can be determined from the effective contact angl...
متن کاملAqueous two-phase polymer partitioning of lipid vesicles of defined size and composition.
The kinetics of the partitioning of lipid vesicles containing acidic phospholipids in aqueous two-phase polymer systems are dependent upon the vesicle size; the larger the vesicles, the more readily they absorb to the interfaces between the two polymer phases and hence are cleared from the top phase as phase separation proceeds. The partitioning of neutral lipid vesicles is principally to the b...
متن کاملComplete Budding and Asymmetric Division of Primitive Model Cells To Produce Daughter Vesicles with Different Interior and Membrane Compositions
Asymmetric cell division is common in biology and plays critical roles in differentiation and development. Unicellular organisms are often used as model systems for understanding the origins and consequences of asymmetry during cell division. Although basic as compared to mammalian cells, these are already quite complex. We report complete budding and asymmetric fission of very simple nonliving...
متن کاملSpontaneous tubulation of membranes and vesicles reveals membrane tension generated by spontaneous curvature.
Recent experimental studies on supported lipid bilayers and giant vesicles have shown that uni-lamellar membrane systems can undergo spontaneous tubulation, i.e., can form membrane tubules or nanotubes without the application of external forces. In the case of supported lipid bilayers, the tube formation was induced by the adsorption of antimicrobial peptides. In the case of giant vesicles, spo...
متن کامل